Precision Temperature Control in Gene Sequencing Equipment: Key Technological Analysis

Release time: August 12, 2025

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Precision Temperature Control in Gene Sequencing Equipment: Key Technological Analysis

Core Temperature Requirements in Gene Sequencing

The gene sequencing workflow involves multiple temperature-sensitive steps, including PCR amplification, fluorescent detection, and sequencing-by-synthesis. The following table outlines the stringent temperature control demands:


Sequencing Step

Temperature Range

Allowable Fluctuation

DNA Denaturation

94–98°C

±0.2°C

Primer Annealing

50–65°C

±0.1°C

Polymerase Extension

72°C

±0.3°C



Huajing Thermal Control’s TEJ9-200XEA (Liquid-to-Air Series) and TP22094 (PCR-Specific Module) are engineered to meet these demands, achieving an exceptional temperature stability of ±0.05°C, far exceeding industry standards.


Technological Advantages of Huajing Thermal Control Solutions


  1. Rapid Thermal Response

    • Utilizes patented thermoelectric cooling (TEC) technology with heating/cooling rates up to 5°C/sec, significantly reducing thermal cycling time.

  2. Multi-Zone Independent Control

    • Supports 16-channel independent temperature regulation, ideal for microfluidic chip-based sequencing with multiple reaction zones.

  3. Intelligent Anti-Interference Algorithm

    • Compensates for ambient temperature fluctuations in real time, ensuring long-term operational stability.

  4. Compact & Modular Design

    • Space-efficient architecture for seamless integration into benchtop and portable sequencing systems.


Key Applications in Gene Sequencing

Huajing’s thermal control solutions are widely adopted in:

  • NGS (Next-Generation Sequencing) thermal management for reaction chambers

  • Single-molecule sequencing chip temperature stabilization

  • Portable sequencers with optimized heat dissipation

  • Sample preparation with uniform temperature distribution


Our Microfluidic Series enables direct integration with sequencing chips, providing nanoliter-scale reaction temperature control, making it ideal for third-generation sequencing platforms.


Why Choose Huajing Thermal Control?


  • 10+ years of expertise in biomedical thermal management

  • 200+ successful deployments in gene sequencing instruments

  • End-to-end solutions from modules to complete thermal systems

  • 24/7 technical support for rapid troubleshooting



Take Action Now:


Visit [Huajing Thermal Control’s official website] for detailed product specifications, or contact us at +86 18575222950 for customized solutions. New customers enjoy exclusive discounts—upgrade your sequencing system’s performance today!


Recommended Products:

  • [TEJ9-200XEA Liquid-to-Air Series] – Optimized for high-throughput sequencers

  • [TP22094 PCR Module] – Ultra-fast thermal cycling for amplification

  • [Microfluidic Thermal Controller] – Chip-level precision for nanopore sequencing


Contact us: sale02@hj-tc.com



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